Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

Article Snippet: Following three washes in Dulbecco's PBS (DPBS, 1X), a fluorescein isothiocyanate (FITC) (excitation 460–500 nm; emission 512–542 nm) conjugated Ab (711‐095‐152; Jackson ImmunoResearch, Cambridge, UK) was used for 1 h at 37°C.

Techniques: Permeability, Diffusion-based Assay, In Vitro, Fluorescence, Imaging, Software, Expressing